[ACÁCIO AGOSTINHO GONÇALVES RODRIGUES, ANA SOFIA QUINTA E COSTA DE OLIVEIRA MORAIS, ANA TERESA PINTO E SILVA, CIDÁLIA IRENE AZEVEDO PINA VAZ, ISABEL CRISTINA SANTOS SILVA DE FARIA RAMOS ANTUNES, RITA MAFALDA DOS SANTOS ROCHA DO ROSÁRIO] PT

[AZEVEDO PINA VAZ, Cidália Irene, AGOSTINHO GONÇALVES RODRIGUES, Acácio, SANTOS SILVA DE FARIA RAMOS ANTUNES, Isabel Cristina, DOS SANTOS ROCHA DO ROSÁRIO, Rita Mafalda, QUINTA E COSTA DE OLIVEIRA MORAIS, Ana Sofia, PINTO E SILVA, Ana Teresa] Gabinete UPIN Praça Gomes Teixeira 4º andar Sala 419, 4099-002 Porto, PT

[CIDÁLIA IRENE AZEVEDO PINA VAZ, ACÁCIO AGOSTINHO GONÇALVES RODRIGUES, RITA MAFALDA DOS SANTOS ROCHA DO ROSÁRIO, ANA SOFIA QUINTA E COSTA DE OLIVEIRA MORAIS, ANA TERESA PINTO E SILVA, ISABELCRISTINA SANTOS SILVA DE FARIA RAMOS ANTUNES] PT

[RODRIGUES ACÁCIO AGOSTINHO GONÇALVES, MORAIS ANA SOFIA QUINTA E COSTA DE OLIVEIRA, SILVA ANA TERESA PINTO E, VAZ CIDÁLIA IRENE PINA, ANTUNES ISABEL CRISTINA SANTOS SILVA DE FARIA RAMO, ROSÁRIO RITA MAFALDA DOS SANTOS ROCHA DO] PT

[AZEVEDO PINA VAZ, Cidália Irene, AGOSTINHO GONÇALVES RODRIGUES, Acácio, SANTOS SILVA DE FARIA RAMOS ANTUNES, Isabel Cristina, DOS SANTOS ROCHA DO ROSÁRIO, Rita Mafalda, QUINTA E COSTA DE OLIVEIRA MORAIS, Ana Sofia, PINTO E SILVA, Ana Teresa] Gabinete UPIN Praça Gomes Teixeira 4º andar Sala 419, 4099-002 Porto, PT

[AZEVEDO PINA VAZ CIDÁLIA IRENE, AGOSTINHO GONÇALVES RODRIGUES ACÁCIO, SANTOS SILVA DE FARIA RAMOS ANTUNES ISABEL CRISTINA, DOS SANTOS ROCHA DO ROSÁRIO RITA MAFALDA, QUINTA E COSTA DE OLIVEIRA MORAIS ANA SOFIA, PINTO E SILVA ANA TERESA] PT

[Cidáia Irene Azevedo Pina Vaz, Acácio Agostinho Gonçalves Rodrigues, Isabel Cristina Santos Silva De Faria Ramos Antunes, Rita Mafalda Dos Santos Rocha Do Rosário, Ana Sofia Quinta E Costa De Oliveira Morais, Ana Teresa Pinto E Silva] PT Porto The present invention relates to a method for detecting resistant microorganisms to a therapeutic agent in a biological sample, comprising the following steps: a. inoculate the said sample, uncultured, on a first tube with, and on a second tube without, at least one therapeutic agent; preferentially further including at least one lysing agent and/or a buffer, and/or a suitable culture medium, or put the sample on a separation serum tube; and incubated it; b. add to both tubes a fluorescent marker; c. perform a fluorescence analysis for obtaining one or more fluorescence or growth parameters for each of the two tubes;wherein the microorganisms resistant phenotype of the biological sample to said therapeutic agent is obtained by comparing the one or more fluorescence parameters between the two tubes. Therefore, the present invention is useful in laboratory procedures or routines for the detection of the susceptibility of different microorganisms to a therapeutic agent, to the determination of microorganisms resistance mechanisms or even to evaluate the amount of antimicrobial drug in the biological sample.

[AZEVEDO PINA VAZ, Cidália Irene, AGOSTINHO GONÇALVES RODRIGUES, Acácio, SANTOS SILVA DE FARIA RAMOS ANTUNES, Isabel Cristina, DOS SANTOS ROCHA DO ROSÁRIO, Rita Mafalda, QUINTA E COSTA DE OLIVEIRA MORAIS, Ana Sofia, PINTO E SILVA, Ana Teresa] Gabinete UPIN, Praça Gomes Teixeira 4º andar, sala 419 P-4099-002 Porto;Faculdade de Medicina da Universidade do Porto, Alameda Professor Hernani Monteiro P-4200-319 Porto;;Faculdade de Medicina da Universidade do Porto, Alameda Professor Hernani Monteiro P-4200-319 Porto;Faculdade de Medicina da Universidade do Porto, Alameda Professor Hernani Monteiro P-4200-319 Porto;Faculdade de Medicina da Universidade do Porto, Alameda Professor Hernani Monteiro P-4200-319 Porto;Faculdade de Medicina da Universidade do Porto, Alameda Professor Hernani Monteiro, P-4200-319 Porto The present invention relates to a method for detecting resistant microorganisms to a therapeutic agent in a biological sample, comprising the following steps: a. inoculate the said sample, uncultured, on a first tube with, and on a second tube without, at least one therapeutic agent; preferentially further including at least one lysing agent and/or a buffer, and/or a suitable culture medium, or put the sample on a separation serum tube; and incubated it; b. add to both tubes a fluorescent marker; c. perform a fluorescence analysis for obtaining one or more fluorescence or growth parameters for each of the two tubes; wherein the microorganisms resistant phenotype of the biological sample to said therapeutic agent is obtained by comparing the one or more fluorescence parameters between the two tubes. Therefore, the present invention is useful in laboratory procedures or routines for the detection of the susceptibility of different microorganisms to a therapeutic agent, to the determination of microorganisms resistance mechanisms or even to evaluate the amount of antimicrobial drug in the biological sample.