[Lynn M. Abell, Stephen M. Allen, Karlene H. Butler, Rebecca E. Cahoon, Howard P. Hershey, Emil M. Orozco] US DE Wilmington This invention relates to isolated nucleic acid fragments encoding phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerases, or “HisA enzymes”. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the HisA enzyme, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the HisA enzyme in a transformed host cell.

[Dams, Thomas, R., Anderson, Paul, C., Daines, Richard, J., Gordon-Kamm, William, J., Kausch, Albert, P., Mackey, Catherine, J., Orozco, Emil, M., Jr., Orr, Peter, M., Stephens, Michael, A.] 3100 Sycamore Road, DeKalb, IL 60115, US This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the ＜i＞Streptomyces bar＜/i＞ gene introduced by microprojectile bombardment into embryonic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen. ＜img id="iaf01" file="imgaf001.tif" wi="78" he="76" img-content="drawing" img-format="tif" /＞

[ADAMS THOMAS R, ANDERSON PAUL C, DAINES RICHARD J, GORDON-KAMM WILLIAM J, KAUSCH ALBERT P, MACKEY CATHERINE J, OROZCO EMIL M, ORR PETER M, STEPHENS MICHAEL A] US

[ADAMS THOMAS R, ANDERSON PAUL C, DAINES RICHARD J, GORDON-KAMM WILLIAM J, KAUSCH ALBERT P, MACKEY CATHERINE J, OROZCO EMIL M JR, ORR PETER M, STEPHENS MICHAEL A] US This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryonic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

[Stephen M. Allen, Perry G. Caimi, Emil M. Orozco, Jr., Mitchell C. Tarczynski, J. Antoni Rafalski] DE Wilmington This invention relates to an isolated nucleic acid fragment encoding a sucrose phosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sucrose phosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sucrose phosphate synthase in a transformed host cell.

[ADAMS THOMAS R, DAINES RICHARD J, KAUSCH ALBERT P, OROZCO EMIL M J R, STEPHENS MICHAEL A, ANDERSON PAUL C, GORDON-KAMM WILLIAM J, MACKEY CATHERINE J, ORR PETER M]

[DAMS THOMAS R, ANDERSON PAUL C, DAINES RICHARD J, GORDON-KAMM WILLIAM J, KAUSCH ALBERT P, MACKEY CATHERINE J, OROZCO EMIL M JR, ORR PETER M, STEPHENS MICHAEL A]

[Stephen M. Allen, Emil M. Orozco, Jr., Anthony J. Kinney, Guo-Hua Miao] US DE Wilmington This invention relates to an isolated nucleic acid fragment encoding a biotin synthases. The invention also relates to the construction of a chimeric gene encoding all or a portion of the biotin synthases, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the biotin synthases in a transformed host cell.

[CAHOON, Rebecca, E., KLEIN, Theodore, M., ODELL, Joan, T., OROZCO, Emil, M., Jr.] 1007 Market Street, WilmingtonDelaware 19898, US

[ADAMS THOMAS R, DAINES RICHARD J, KAUSCH ALBERT P, OROZCO EMIL M JR, STEPHENS MICHAEL A, ANDERSON PAUL C, GORDON-KAMME WILLIAM J, MACKEY CATHERINE J, ORR PETER M]