[LIU WENHAO, HUANG JINMING, WEI XIAOCHAO, YANG CHUNHONG, WANG JINPENG, JU ZHIHUA, WANG XIUGE, JIANG QIANG, ZHANG YARAN, GAO YAPING, WANG LINGLING, GAO YUNDONG] CN;CN Absract The present invention provides a method for screening a bovine high-altitude hypoxic adaptation gene ALDOC and a functional molecular marker and an application thereof, and belongs to the technical field of molecular breeding of animals. The present invention selects cattle breeds distributed at high- and low-altitude from the perspectives of bovine genomic selection and genetic adaptation, integrates three genomic selection signature analysis methods including FLK, hapFLK and XPEHH by using genotyping data from high-density SNP chip, compares genomic selection signatures between the high- and low-altitude cattle breeds and within the cattle breeds, identifies candidate genes that adapt to extreme high-altitude hypoxic environments by combining screening strategies and bioinformatic analysis, further mines potential functional molecular markers by selection signature verification and re-sequencing data analysis, and establishes corresponding test methods. The present invention provides a scientific basis and a simple and easy test technology for molecular breeding such as breeding of high-altitude hypoxic characteristic cattle breeds, and has important significance and value for the protection, evaluation and utilization of local cattle breed genetic resources.

[HUANG, Jinming 黄金明, ZHAO, Han 赵晗, WANG, Xiuge 王秀革, JU, Zhihua 鞠志花, JIANG, Qiang 姜强, WANG, Jinpeng 王金鹏, ZHANG, Yaran 张亚冉, LIU, Yong 刘勇, WEI, Xiaochao 魏晓超, GAO, Yaping 高亚平, LIU, Wenhao 刘文浩, WANG, Lingling 王玲玲, GAO, Yundong 高运东] No. 159, Industrial North Road, Licheng District 中国山东省济南市历城区工业北路159号, Shandong 250132Jinan, Shandong 250132;No. 159, Industrial North Road, Licheng District 中国山东省济南市历城区工业北路159号, Shandong 250132Jinan, Shandong 250132 Provided are a method for screening a specific molecular marker of cattle adapting to high altitude hypoxia, and use of the specific marker in screening cattle breeds or individuals suitable for survival in high altitude hypoxia. For the genetic characteristics of adaptation of cattle breeds to high altitude hypoxia, from the perspective of genome evolution, selection and adaptation, local cattle breeds distributed at high and low altitudes are selected, and multi-genome-selection-signal and genome-wide association analysis methods and strategies are integrated to screen a key gene and a molecular marker adapting to high altitude hypoxia. The detection method designed according to the key gene and the marker features high accuracy and easy operation during application. By the analysis of cattle breeds at different altitudes, gene ACSS2 related to adaptation to high altitude hypoxia and a haplotype thereof are found, and specific SNP that receives the strongest selection signal on the gene is located. The method has great significance and practical value for cattle molecule breeding. Provided are a method for screening a specific molecular marker of cattle adapting to high altitude hypoxia, and use of the specific marker in screening cattle breeds or individuals suitable for survival in high altitude hypoxia. For the genetic characteristics of adaptation of cattle breeds to high altitude hypoxia, from the perspective of genome evolution, selection and adaptation, local cattle breeds distributed at high and low altitudes are selected, and multi-genome-selection-signal and genome-wide association analysis methods and strategies are integrated to screen a key gene and a molecular marker adapting to high altitude hypoxia. The detection method designed according to the key gene and the marker features high accuracy and easy operation during application. By the analysis of cattle breeds at different altitudes, gene ACSS2 related to adaptation to high altitude hypoxia and a haplotype thereof are found, and specific SNP that receives the strongest selection signal on the gene is located. The method has great significance and practical value for cattle molecule breeding.

[ZHAO SHOUSHAN, DONG JIANZHONG, LIU XIAOQING, SUN HONGXIA, ZHANG ZHIWEI, MA CHAO, ZHANG CHENG, FU ZHAOHUI, XIE QIANCHENG, YANG GUANG, YANG CHANGDONG, DONG HAI, GAO SHAN, GAO XIANBO, YU JINXIA, MA WENHAO, WANG CUIPING, LI XUEYAN, SHI XIAOMEI] CN;CN

[LIU, Chao 刘超, GAO, Peng 高鹏, WANG, Lingfei 王凌飞, GAO, Wenhao 高文浩, LIU, Xiaofeng 刘晓峰, ZHANG, Junzhao 张军召, WU, Chaoyu 吴超瑜] No.20 Haitainan Road, Huayuan New Technology 中国天津市西青区华苑产业区海泰南道20号, Tianjin 300384Industry Development Area, Xiqing District, TianjinTianjin 300384 Disclosed is a light-emitting diode for emitting green-yellow light, comprising a first-type semiconductor layer, an active layer and a second-type semiconductor layer, characterized in that: the active layer sequentially includes a first quantum well, a second quantum well, a strain compensation layer, and a third quantum well, wherein the first quantum well is constructed as a strain-free quantum well, and the second and third quantum wells are strained quantum wells. In the present invention, a strain-free quantum well and a large-strain quantum well are combined, and a strain compensation measure is used to prevent lattice relaxation, reducing the component of Al so that a device is more reliable, and also improving the limiting effect of a quantum well on a carrier and thus improving the internal quantum efficiency.

[HUANG JINMING, ZHAO HAN, WANG XIUGE, JU ZHIHUA, JIANG QIANG, WANG JINPENG, ZHANG YARAN, LIU YONG, WEI XIAOCHAO, GAO YAPING, LIU WENHAO, WANG LINGLING, GAO YUNDONG] CN;CN Provided are a method for screening a specific molecular marker of cattle adapting to high altitude hypoxia, and use of the specific marker in screening cattle breeds or individuals suitable for survival in high altitude hypoxia. For the genetic characteristics of adaptation of cattle breeds to high altitude hypoxia, from the perspective of genome evolution, selection and adaptation, local cattle breeds distributed at high and low altitudes are selected, and multi-genome-selection-signal and genome-wide association analysis methods and strategies are integrated to screen a key gene and a molecular marker adapting to high altitude hypoxia. The detection method designed according to the key gene and the marker features high accuracy and easy operation during application. By the analysis of cattle breeds at different altitudes, gene ACSS2 related to adaptation to high altitude hypoxia and a haplotype thereof are found, and specific SNP that receives the strongest selection signal on the gene is located. The method has great significance and practical value for cattle molecule breeding.

[HUANG, Jinming 黄金明, BAI, Jiachen 白佳琛, JU, Zhihua 鞠志花, JIANG, Qiang 姜强, WANG, Jinpeng 王金鹏, ZHANG, Yaran 张亚冉, WANG, Xiuge 王秀革, WEI, Xiaochao 魏晓超, GAO, Yaping 高亚平, YANG, Chunhong 杨春红, LIU, Wenhao 刘文浩, LI, Rongling 李荣岭, GAO, Yundong 高运东] No.202, Industrial North Road, Licheng District, 中国山东省济南市历城区工业北路202号, Shandong 250132Jinan, Shandong 250132;No.202, Industrial North Road, Licheng District, 中国山东省济南市历城区工业北路202号, Shandong 250132Jinan, Shandong 250132 Provided are a method for screening ketosis-resistant molecular markers of dairy cows and an application thereof, which belong to the technical field of molecular genetic biology. Taking healthy and ketosis dairy cows as samples, chip sequencing, data filling, selection signal analysis, and whole-genome association analysis, and the like are performed to screen out a key gene APOA1 that affects the resistance of Chinese Holstein cattle to ketosis. The single nucleotide polymorphism site g.-572A＞G is obtained by screening in the APOA1 gene promoter region. By means of association analysis, promoter activity analysis and other methods, it is obtained that the concentration of β-hydroxybutyric acid in the blood of GG genotype dairy cows is significantly lower than that of the AA genotype, and the promoter activity of GG genotype individuals is significantly higher than that of AA genotype individuals. By using the technical solution, genotyping the APOA1 gene promoter region site g.-572A＞G may screen out dairy cow individuals that have strong ketosis resistance, thus achieving the purpose of saving on breeding costs. Provided are a method for screening ketosis-resistant molecular markers of dairy cows and an application thereof, which belong to the technical field of molecular genetic biology. Taking healthy and ketosis dairy cows as samples, chip sequencing, data filling, selection signal analysis, and whole-genome association analysis, and the like are performed to screen out a key gene APOA1 that affects the resistance of Chinese Holstein cattle to ketosis. The single nucleotide polymorphism site g.-572A＞G is obtained by screening in the APOA1 gene promoter region. By means of association analysis, promoter activity analysis and other methods, it is obtained that the concentration of β-hydroxybutyric acid in the blood of GG genotype dairy cows is significantly lower than that of the AA genotype, and the promoter activity of GG genotype individuals is significantly higher than that of AA genotype individuals. By using the technical solution, genotyping the APOA1 gene promoter region site g.-572A＞G may screen out dairy cow individuals that have strong ketosis resistance, thus achieving the purpose of saving on breeding costs.

[HUANG JINMING, WANG XIUGE, WEI XIAOCHAO, JU ZHIHUA, JIANG QIANG, YANG CHUNHONG, ZHANG YARAN, GAO YAPING, WANG JINPENG, LIU WENHAO, DAI YUNPING, GAO YUNDONG] CN;CN Abstract The present invention discloses a set of primer combination and kit for simultaneously detecting 93 bovine genetic defect genes and lethal haplotypes. Through this set of primers and kit, causal mutation sites (SNP, insertion and deletion of short fragments) of 93 genetic defect genes (such as PIRM syndrome, curved tail syndrome, and epidermolysis bullosa) and lethal haplotypes (HH1, HH3, HH4, HH5, and JH1) can be detected at one time, and carriers with single genetic defect genes and lethal haplotype carriers are screened out, so as to provide accurate guidance for breeding and genetic evaluation of breeding cattle, selection and mating of cattle, genetic improvement of populations, new breed cultivation, and protection of genetic resources. The present invention has the characteristics of high throughput, low cost, high accuracy, and the like, is suitable for beef cattle and cow breeds, and can be widely used in the field of cattle breeding and reproduction.

[HUANG JINMING, ZHAO HAN, WANG XIUGE, JU ZHIHUA, JIANG QIANG, WANG JINPENG, ZHANG YARAN, LIU YONG, WEI XIAOCHAO, GAO YAPING, LIU WENHAO, WANG LINGLING, GAO YUNDONG] ; Provided are a method for screening a specific molecular marker of cattle adapting to high altitude hypoxia, and use of the specific marker in screening cattle breeds or individuals suitable for survival in high altitude hypoxia. For the genetic characteristics of adaptation of cattle breeds to high altitude hypoxia, from the perspective of genome evolution, selection and adaptation, local cattle breeds distributed at high and low altitudes are selected, and multi-genome-selection-signal and genome-wide association analysis methods and strategies are integrated to screen a key gene and a molecular marker adapting to high altitude hypoxia. The detection method designed according to the key gene and the marker features high accuracy and easy operation during application. By the analysis of cattle breeds at different altitudes, gene ACSS2 related to adaptation to high altitude hypoxia and a haplotype thereof are found, and specific SNP that receives the strongest selection signal on the gene is located. The method has great significance and practical value for cattle molecule breeding.

[ZHANG, Huafeng 张华凤, GAO, Ping 高平, MA, Wenhao 马文豪, SUN, Yuchen 孙愉宸] {Institute of Health and Medicine, Hefei comprehensive National Science Center. No.4090, Susong road, Economic and Technological Development Zone 中国安徽省合肥市经济技术开发区宿松路4090号合肥综合性国家科学中心大健康研究院, Anhui 230601Hefei, Anhui 230601;CN CN(CN)(CN)} Provided is use of an OXCT1 protein as a succinyltransferase. The OXCT1 protein as the succinyltransferase can promote the growth of cancer cells by means of regulating and controlling protein succinylation modification; the activity of the succinyltransferase can be inhibited by means of designing related enzyme inhibitors, so that the development and progression of cancers are inhibited, thus providing a new thought for subsequent targeted therapy.

[LI BINYONG, DU ZEYAN, GAO JIAQI, YANG ZILAN, LI WENHAO] CN