[Jefferis, Royston, Steensgaard, Jens, c/o University of Aarhus] Unilever House Blackfriars P.O. Box 68, London EC4P 4BQ, GB An antigen/antibody precipitate is obtained, using monoclonal antibodies, the monoclonal antibodies (samples I or li or III or IV) being selected so as to be specific to two distinct antigenic binding sites (L or C 2 or C3) on a protein (IgG) in a sample under test. The proportions of sub-populations of immunoglobulins (IgG kappa, IgG lambda) in a sample is determined by reacting the sample with a combination of antibodies (II and IV) both of which are specific to the heavy chains (H) of both sub-populations (IgG kappa, IgG lambda) and reacting the sample with an antibody combination (I and II) specific to said heavy chain (H) and to an antigenic determinant expressed by only one (IgG kappa) of the sub-populations.＜img id="iaf01" file="imgaf001.tif" wi="76" he="78" img-content="drawing" img-format="tif" inline="no" /＞

[JEFFERIS, Royston, COBBOLD, Mark, PLANT, Timothy, GOODALL, Delia Margaret, DRAYSON, Mark Trehane] GB,Edgbaston Birmingham West Midlands B15 2TT,;

[Jefferis Royston(Birmingham,GB2), Steensgaard Jens(Aarhus,DKX)] An antigen/antibody precipitate is obtained, using monoclonal antibodies, the monoclonal antibodies (samples I or II or III or IV) being selected so as to be specific to two distinct antigenic binding sites (L or C 2 or C 3) on a protein (IgG) in a sample under test. The proportions of sub-populations of immunoglobulins (IgG kappa, IgG lambda) in a sample is determined by reacting the sample with a combination of antibodies (II and IV) both of which are specific to the heavy chains (H) of both sub-populations (IgG kappa, IgG lambda) and reacting the sample with an antibody combination (I and II) specific to said heavy chain (H) and to an antigenic determinant expressed by only one (IgG kappa) of the sub-populations.

[JEFFERIS ROYSTON, STEENSGAARD JENS] GB

[JEFFERIS, Royston, COBBOLD, Mark, PLANT, Timothy, GOODALL, Delia Margaret, DRAYSON, Mark Trehane] Edgbaston Birmingham B15 2TT;40 Kensington Road SeIIy Park Birmingham West Midlands B29 7LW;48 First Avenue SeIIy Park Birmingham West Midlands B29 7NS;4 Saintbury Drive Solihull West Midlands B91 3SZ;18 Diddington Lane Hampton in Arden Solihull West Midlands B92 OBZ;Treworld Minster Boscastle Cornwall PL35 OBW The present invention provides improved methods for measuring the ratio of free K immunoglobulin light chain molecules to free λ immunoglobulin light chain molecules in a test sample, using a monoclonal antibody that specifically binds to free K and a monoclonal antibody that specifically binds to free λ. The application also provides specific monoclonal antibodies that can be used in the method of the invention, as well as kits containing said monoclonal antibodies.

[Royston Jefferis, Jens Steensgaard] GB2 Birmingham An antigen/antibody precipitate is obtained, using monoclonal antibodies, the monoclonal antibodies (samples I or II or III or IV) being selected so as to be specific to two distinct antigenic binding sites (L or C 2 or C 3) on a protein (IgG) in a sample under test. The proportions of sub-populations of immunoglobulins (IgG kappa, IgG lambda) in a sample is determined by reacting the sample with a combination of antibodies (II and IV) both of which are specific to the heavy chains (H) of both sub-populations (IgG kappa, IgG lambda) and reacting the sample with an antibody combination (I and II) specific to said heavy chain (H) and to an antigenic determinant expressed by only one (IgG kappa) of the sub-populations.

[Royston Jefferis, Mark Cobbold, Timothy Plant, Delia Margaret Goodall, Mark Trehane Drayson] GB Birmingham The present invention provides improved methods for measuring the ratio of free K immunoglobulin light chain molecules to free λ immunoglobulin light chain molecules in a test sample, using a monoclonal antibody that specifically binds to free K and a monoclonal antibody that specifically binds to free λ. The application also provides specific monoclonal antibodies that can be used in the method of the invention, as well as kits containing said monoclonal antibodies.

[JEFFERIS, Royston, STEENSGAARD, Jen] An antigen/antibody precipitate is obtained, using monoclonal antibodies, the monoclonal antibodies (samples I or II or III or IV) being selected so as to be specific to two distinct antigenic binding sites (L or C2 or C3) on a protein (IgG) in a sample under test. The proportions of sub-populations of immunoglobulins (IgG kappa, IgG lambda) in a sample is determined by reacting the sample with a combination of antibodies (II and IV) both of which are specific to the heavy chains (H) of both sub-populations (IgG kappa, IgG lambda) and reacting the sample with an antibody combination (I and II) specific to said heavy chain (H) and to an antigenic determinant expressed by only one (IgG kappa) of the sub-populations.