[YE ZHOU, LV DANYA, GE WEN, WANG YANGYANG, ZHANG ZHONGLIANG, FAN MENGYU, LI YAQI, ZHU ZIHUI] CN

[LV, Meng 吕猛, GUO, Mengyu 郭孟宇, GUO, Lei 郭磊, WU, Xiaopeng 吴晓鹏, CHEN, Xianyong 陈仙勇] No. 5 Building, Wispark 1 中国天津市滨海高新区海泰发展六道3号星企中心5号楼, Tianjin 300384No. 3 Hi-Tech Development Avenue 6, Binhai Hi-tech AreaTianjin 300384 Provided is an apparatus for detecting a defect of a laser welding protection lens on an automobile production line. The apparatus comprises: a coaxial light source (10) for emitting collimated probe light, the direction of the probe light being perpendicular to a pre-set horizontal direction; a transflective mirror (20) arranged above the coaxial light source (10) and used for reflecting the probe light to a pre-set position; a reflecting mirror (30) for receiving the light reflected by the transflective mirror (20) and reflecting the light reflected by the transflective mirror (20) to a protection lens; an industrial camera (40) parallel to the transflective mirror (20) and the reflecting mirror (30) in the pre-set horizontal direction and used for receiving incident light to obtain a detected image, wherein the incident light is incident light formed after the probe light is reflected by the protection lens and then passes through the reflecting mirror (30) and the transflective mirror (20); and a processor (50) for performing calculation, according to the detected image, to determine whether a dot defect exists on the protection lens. Further provided is a method for detecting a defect of a laser welding protection lens by using the apparatus. Provided is an apparatus for detecting a defect of a laser welding protection lens on an automobile production line. The apparatus comprises: a coaxial light source (10) for emitting collimated probe light, the direction of the probe light being perpendicular to a pre-set horizontal direction; a transflective mirror (20) arranged above the coaxial light source (10) and used for reflecting the probe light to a pre-set position; a reflecting mirror (30) for receiving the light reflected by the transflective mirror (20) and reflecting the light reflected by the transflective mirror (20) to a protection lens; an industrial camera (40) parallel to the transflective mirror (20) and the reflecting mirror (30) in the pre-set horizontal direction and used for receiving incident light to obtain a detected image, wherein the incident light is incident light formed after the probe light is reflected by the protection lens and then passes through the reflecting mirror (30) and the transflective mirror (20); and a processor (50) for performing calculation, according t

[Haibo Wang, Mengyu Lv, Fushuang Dong, Yanmin Zhang, Zhiheng Ren, Fan Yang, Guozhong Sun, Xian Xu, Xueping Shi, Huanhuan Zhang, He Zhao, Jianfang Chai, Yiping Gao, Yongwei Liu, Jinyong Zhu, Qiufen Han, Qiang Zhang, Huijie Ma, Zhanwu Wang, Junfeng Guan] CN Shijiazhuang The present invention is a method of shoot apical meristem transformation for dicot plant via sufficient and micro wounding (SMW). The technical process includes: germinate the seeds in Petri dish or in the nutrient matrix which can be transplanted with the seedling; expose the shoot apical meristem by removing one cotyledon away; make sufficient and micro wounding transformation treatment in vivo to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; after co-cultivation, develop the treated objects directly to normal plants in the nutrient matrix and then transplant the matrix and seedling together into pot or field; promote the plant to develop more branches, pods, bolls, fruits, and seeds; harvest the seeds of each branch of individual T0 plants separately; detect and identify the transformation results in T1 generation from every T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, no need to graft, unnecessary to carry resistant marker, high survival rate in transplantation and seedlings recovered rapidly, easy to manipulate and large scale to perform, and applicable to all dicot plants which can set seeds. The transformation efficiencies for cotton and soybean using this method are 50% and 76.5%, respectively.

[WANG, Haibo, DONG, Fushuang, LV, Mengyu, ZHANG, Yanmin, REN, Zhiheng, YANG, Fan, LIANG, Xinchao, ZUO, Wenbo, SHI, Xueping, ZHANG, Huanhuan, GAO, Yiping, ZHAO, He, XU, Xian, SUN, Guozhong, CHAI, Jianfang, LIU, Yongwei, ZHU, Jinyong, HAN, Qiufen, ZHANG, Qiang, MA, Huijie, WANG, Zhanwu, GUAN, Junfeng] No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051;No.598, Hepingxilu Shijiazhuang, Hebei 050051 The present invention provides a monocotyledon transgenic method for invading growing points of seed buds minimally and fully, comprising: germinating seeds, and when buds extend to be 0.2 to 2 centimeters long, removing coleoptile to expose growing points; pricking and brushing the growing points by using a minimally-invasive brush of which the diameter of a single brush hair is 4 to 20 micrometers, the exposed length is 0.5 to 3 millimeters and the number of the brush hairs is 100 to 5000 and which is dipped with agrobacterium mediated conversion liquid; and performing identification on T1 generation. This present invention is applicable to all seed bearing monocotyledons. A conversion effect that the genetic conversion rate of wheat is 49%, the genetic conversion rate of paddy is 66.3% and the genetic conversion rate of corn is 100% is achieved.

[ZHANG, Xiaowei 张笑薇, LV, Jinli 吕金丽, ZHAO, Shaowei 赵少伟, GAO, Mengyu 高蒙玙, ZOU, Yuanqiang 邹远强, XIAO, Liang 肖亮] Mainbuilding, Beishan Industrial Zone, Yantian Street, Yantian District 中国广东省深圳市盐田区盐田街道北山工业区综合楼, Guangdong 518083Shenzhen, Guangdong 518083 Provided are a lactobacillus crispatus strain for preventing and/or treating genital tract flora disorder related diseases, and an application thereof. The lactobacillus crispatus strain is lactobacillus crispatus LG55-27 obtained by screening, and the lactobacillus crispatus LG55-27 can generate secretions such as lactic acid and hydrogen peroxide to inhibit the growth of pathogenic bacteria of genital tract infection, and is used for preventing and/or treating the genital tract flora disorder related diseases.

[Haibo Wang, Fushuang Dong, Mengyu Lv, Yanmin Zhang, Zhiheng Ren, Fan Yang, Xinchao Liang, Wenbo Zuo, Xueping Shi, Huanhuan Zhang, Yiping Gao, He Zhao, Xian Xu, Guozhong Sun, Jianfang Chai, Yongwei Liu, Jinyong Zhu, Qiufen Han, Qiang Zhang, Huijie Ma, Zhanwu Wang, Junfeng Guan] CN Shijiazhuang The present invention is a method of shoot apical meristem transformation for monocot plant via sufficient and micro wounding (SMW). The technical process includes: expose the apical meristem by removing the coleoptile away when the shoot grows to 0.2-2 cm after 1-2 days of seed germination; make sufficient and micro wounding transformation to the apical meristem by stabbing and brushing for 2-3 times using the SMW brush having 100-5000 bristles which is 4-20 μm in diameter for each one and 0.5-3 mm in exposed length, and dipped with the Agrobacterium tumefaciens containing binary vector harboring exogenous genes; develop the treated meristems directly to normal plants after co-cultivation; promote the plants to develop big spikes and set more seeds; harvest the seeds of T0 plants separately; detect and identify the transformation results in T1 generation which is bred from each individual T0 plant. The advantages of the invention are independent of tissue culture, unlimited in genotype, unnecessary to carry resistant marker, simple and large scale to perform, and applicable to all monocot plants which can set seeds. The transformation efficiencies for wheat, rice and maize using this method are 49%, 66.3%, and 100%, respectively.

[Meng Lv, Mengyu Guo, Lei Guo, Xiaopeng Wu, Xianyong Chen] CN Tianjin A device for detecting a defect of a laser welding cover lens includes a coaxial light source configured to emit collimating detection light, wherein the direction of the detection light is perpendicular to a preset horizontal direction; a half transparent and half reflecting mirror arranged above the coaxial light source to reflect the detection light to a preset position; a reflecting mirror configured to reflect light from the half transparent and half reflecting mirror to the cover lens; an industrial camera parallel to the half transparent and half reflecting mirror and the reflecting mirror and configured to receive incident light from the detection light to obtain a detection image, wherein the incident light is reflected by the cover lens and passes through the reflecting mirror and the half transparent and half reflecting mirror; and a processor configured to determine whether there exists a bad point on the cover lens.

[Meng LV, Mengyu Guo, Lei GUO, Xiaopeng WU, Xianyong CHEN] CN Tianjin A device for detecting a defect of a laser welding cover lens includes a coaxial light source configured to emit collimating detection light, wherein the direction of the detection light is perpendicular to a preset horizontal direction; a half transparent and half reflecting mirror arranged above the coaxial light source to reflect the detection light to a preset position; a reflecting mirror configured to reflect light from the half transparent and half reflecting mirror to the cover lens; an industrial camera parallel to the half transparent and half reflecting mirror and the reflecting mirror and configured to receive incident light from the detection light to obtain a detection image, wherein the incident light is reflected by the cover lens and passes through the reflecting mirror and the half transparent and half reflecting mirror; and a processor configured to determine whether there exists a bad point on the cover lens.

[XiaoJun NIU, MengYu LV, Xia LI, Li ZHANG, NingYu TU, XingYao YE, DongQing ZHANG, LiHua CHENG, HuaFang GUO] CN Guangzhou The invention discloses a device for producing biogas with high methane content by utilizing livestock and poultry feces, wherein the interior of a tank body of a biogas fermentation tank is divided by a baffle, so as to form a main reaction chamber and an auxiliary reaction chamber which are communicated in upper portions, so that a reactant flows into the auxiliary reaction chamber only after entering the main reaction chamber via a relatively low feeding hole and then reaching a high position of a liquid level, and extension of fermentation time is realized, meanwhile, scales formed at the top of fermentation broth flow into the auxiliary reaction chamber along with liquid, so that the interior of the main reaction chamber keeps a liquid state all the time, and sealing and reduction of quantity of anaerobic bacteria are avoided.

[WANG, Haibo, LV, Mengyu, DONG, Fushuang, ZHANG, Yanmin, REN, Zhiheng, YANG, Fan, SUN, Guozhong, XU, Xian, SHI, Xueping, ZHANG, Huanhuan, ZHAO, He, CHAI, Jianfang, GAO, Yiping, LIU, Yongwei, ZHU, Jinyong, HAN, Qiufen, ZHANG, Qiang, MA, Huijie, WANG, Zhanwu, GUAN, Junfeng] LV, Mengyu NO.598, Hepingxilu Shijiazhuang, Hebei 050051;NO.598, Hepingxilu Shijiazhuang, Hebei 050051;;;;;;;;;;;;;;;;;;; The present invention provides a dicotyledon transgenic method for invading growing points of seed sprouts or seedling stems minimally and fully, comprising: removing a cotyledon to expose growing points, and pricking and brushing the growing points by using a minimally-invasive brush of which the diameter of a single brush hair is 4 to 20 micrometers, the length of the brush hair is 0.5 to 3 millimeters and the number of the brush hairs is 100 to 5000 and which is dipped with agrobacterium mediated conversion liquid; and performing identification on T1 generation. The present invention is applicable to all seed bearing dicotyledons. A conversion effect that the conversion rate of cotton is 50%, and the conversion rate of soybeans is 76.5% during the conversion of the cotton and the soybeans is achieved.