[ZHONG, Jianying 钟建英, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, LI, Shaohua 李少华, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, LI, Shaohua 李少华, ZHANG, Wentao 张文涛, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, LI, Shaohua 李少华, ZHANG, Wentao 张文涛, KOU, Xinmin 寇新民, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, LI, Shaohua 李少华, ZHANG, Wentao 张文涛, KOU, Xinmin 寇新民, LIU, Yifan 刘逸凡, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, LI, Shaohua 李少华, ZHANG, Wentao 张文涛, KOU, Xinmin 寇新民, LIU, Yifan 刘逸凡, MAO, Zhikuan 毛志宽, ZHONG, Jianying 钟建英, ZHANG, Yiming 张一茗, SUN, Yinshan 孙银山, YIN, Junhua 尹军华, WANG, Zhen 王振, HAO, Sha 郝莎, SONG, Yakai 宋亚凯, GAO, Qunwei 高群伟, LI, Shaohua 李少华, ZHANG, Wentao 张文涛, KOU, Xinmin 寇新民, LIU, Yifan 刘逸凡, MAO, Zhikuan 毛志宽, MA, Dongling 马东岭] No. 86, West Chang'an Street, Xicheng District 中国北京市西城区西长安街86号, Beijing 100031Beijing 100031;No.22, Nanhuandong Road 中国河南省平顶山市南环东路22号, Henan 467001Pingdingshan, Henan 467001;No.22, Nanhuandong Road 中国河南省平顶山市南环东路22号, Henan 467001Pingdingshan, Henan 467001 A method of calculating a step voltage and a maximum contact voltage in a gas-insulated substation, comprising: upon precisely calculating a housing loop current condition, a grounding condition and a housing potential distribution condition of a totally-enclosed combined electric apparatus, obtaining a potential distribution condition of a soil surface, thereby obtaining, on the basis of the above, distribution conditions of the substation step voltage and a maximum contact voltage. The method greatly increases calculation precision, more precisely performs calculations with respect to a problem at the substation, and performs targeted and effective prevention and maintenance with respect to problems resulting from faults at the substation. The method is economically favorable, and reduces unnecessary economic losses by targeted prevention.

[ZHANG, Dazhun 张大准, XIAO, Chuan 肖川, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054China Hi-tech Building, Yuelingwan Rd, NanshanShenzhen, Guangdong 518054 The present invention relates to the technical field of biological detection, and provides an anti-torch-IgG antibody profile chip and a preparation method therefor, and a TORCH detection kit and a detection method. The anti-torch-IgG antibody profile chip can be used for simultaneously and quantitatively detecting the infection of related pathogenic microbes of TORCH. Moreover, by optimizing coating liquid and coating conditions of the chip and sealing stabilizing agents, the stability of the anti-torch-IgG antibody profile chip is better, and the service cycle is longer. The TORCH detection kit provided by the present invention has the advantages that the consumption of relevant antigens can be effectively reduced, the antigen cost can be reduced, and meanwhile, the stable period is longer, the use period is longer, the transportation cost is reduced, and the storage and transportation convenience is improved. The TORCH detection method can be used for simultaneously and quantitatively detecting the infection of various related pathogenic microbes of TORCH, the sensitivity is high, and the specificity is high. The present invention relates to the technical field of biological detection, and provides an anti-torch-IgG antibody profile chip and

[ZHANG, Dazhun 张大准, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 China Hi-tech Building 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054Yuelingwan Rd, NanshanShenzhen, Guangdong 518054 Disclosed are a composition for an enzyme-linked immunosorbent assay kit, a diabetes antibody repertoire detection kit and a preparation method therefor. The composition comprises a blocking liquid and an ELISA diluent; the blocking liquid contains BSA, polyvinyl alcohol, mannitol, sodium azide and disodium hydrogen phosphate; and the ELISA diluent contains Tris, citric acid, BSA, polyethylene glycol, acacia gum, betaine and Proclin 300. The enzyme-linked immunosorbent assay kit can maintain the stability of detection for a long time. In addition, based on the better stability, the diabetes detection kit prepared by the composition further can accurately diagnose and type diabetes, and particularly with regard to IAA detection, a higher positive rate is achieved. Disclosed are a composition for an enzyme-linked immunosorbent assay kit, a diabetes antibody repertoire detection kit and a preparation method therefor. The composition com

[ZHANG, Dazhun 张大准, XIONG, Zuying 熊祖应, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 China Hi-tech Building 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054Yuelingwan Rd, NanshanShenzhen, Guangdong 518054 Disclosed are a composition, a chip and a preparation method for the chip and a detection device including the chip. The detection device has high sensitivity and specificity, can detect 12 antibodies at a time, has the advantages of high accuracy, convenient operation, saving on antigens, cost reduction, etc., and also optimizes related reagent formulations and treatment methods, and makes the stability of a kit better and storage and transportation conditions more convenient. Disclosed are a composition, a chip and a preparation method for the chip and a detection device including the chip. The detection device has high

[ZHANG, Dazhun 张大准, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 China Hi-tech Building, No.2076 Yuelingwan Rd., Nanshan 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054Shenzhen, Guangdong 518054 Disclosed are a biotinylated insulin antigen having a structure as represented by formula 1 and a biotinylation process thereof. The biotinylated insulin antigen of the present invention improves the coating efficiency of an insulin antigen because it is amplified by pre-coated avidin, can significantly improve the sensitivity of an insulin antibody detection reagent or kit prepared thereby, improves the positive rate of insulin antibody detection, and reduces the probability of missed detection or false detection.

[ZHANG, Dazhun 张大准, XIAO, Chuan 肖川, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 China Hi-tech Building 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054Yuelingwan Rd, NanshanShenzhen, Guangdong 518054 The present invention belongs to the field of biotechnology, and discloses an anti-ToRCH-IgM antibody repertoire chip, a preparation method therefor, a TORCH detection kit and a detection method. The anti-ToRCH-IgM antibody repertoire chip is coated with protein chips of antigens of pathogenic microorganisms related to TORCH detection, and can be used for simultaneously and quantitatively detecting the infection of various related pathogenic microorganisms of TORCH. The ToRCH detection kit includes the anti-ToRCH-IgM antibody repertoire chip.

[ZHANG, Dazhun 张大准, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 China Hi-tech Building 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054Yuelingwan Rd, NanshanShenzhen, Guangdong 518054 Disclosed are a composition for an enzyme-linked immunosorbent assay kit, an anti-nuclear antibody repertoire detection kit and a preparation method therefor. The composition of the present invention comprises a blocking liquid and an ELISA diluent; the blocking liquid contains casein, sucrose, PBS, mannitol, Tween 20, betaine and sodium azide; and the ELISA diluent contains PBS, casein, sodium p-hydroxybenzoate, PEG, betaine and Proclin 300. Disclosed are a composition for an enzyme-linked immunosorbent assay kit, an anti-nuclear antibody repertoire detection kit and a preparation method therefor. The composition of the present invention comprises a blocking liquid and an ELISA diluent; the blocking liquid contains casein, sucrose, P

[ZHANG, Dazhun 张大准, ZHANG, Yongding 张永顶, MA, Weimin 马伟民, WANG, Hongtao 王洪涛, MA, Xinmin 马新民] 6-A2 中国广东省深圳市南山区月亮湾大道2076号中国高科大厦六楼A2, Guangdong 518054China Hi-tech Building, Yuelingwan Rd, NanshanShenzhen, Guangdong 518054 Provided are a composition for an enzyme-linked immunosorbent assay kit, and a tumor marker test kit and a preparation method therefor. The composition comprises a blocking buffer and an enzyme label diluent; the blocking buffer comprises fetal bovine serum, trehalose, PBS, Tween20, PVA, and sodium azide; the enzyme label diluent comprises Tris, casein, trehalose, sodium p-hydroxybenzoate, gum arabic, PEG, and Proclin300. Provided are a composition for an enzyme-linked immunosorbent assay kit, and a tumor marker test kit and a preparation method therefor. The composition comprises a blocking buffer and an enzyme label diluent; the blocking buffer comprises fetal bovine

[HE, Xinmin 何新民, MA, Yunchen 马云程, LIANG, Hongwei 梁宏伟, CAO, Fengan 曹逢安, YAO, Caiyun 姚彩云] Room 4101, 1st Floor, Building 1-4, Yard 8, Shangdi West Road, Haidian District 中国北京市海淀区上地西路8号院1-4号楼1层4101, Beijing 100085Beijing 100085 Provided are a complex enzyme cleaning agent and the use thereof. The complex enzyme cleaning agent comprises the following components: 10-55 parts of polyphenol oxidase, 10-30 parts of phytase, 10-40 parts of mannase, 5-40 parts of keratinase, and 5-20 parts of arabinosidase. The complex enzyme cleaning agent is composed of a safe biocatalyst with a specific efficacy, that is, an enzyme preparation, and can efficiently decompose organic adhesive substances remaining on the surface of equipment during a beer brewing process, such as wort, fermentation broth and liquor, rapidly reduce the viscosity, surface tension and adhesive force of dirt and stains, improve the dispersity and permeability, and complete CIP cleaning of production equipment by means of a certain shearing force, at a certain temperature, and within a certain time, thereby achieving the aim of completely replacing strong acid and strong base cleaning agents.

[LI XIAONIAN, ZHANG QUNFENG, CHEN XINMIN, FENG FENG, MA LEI, LU CHUNSHAN, LI CHUNSHENG] CN